Part:BBa_K5232001

Usage and Biology

The 3' end strand has the complementary sequence to primer0 amplified by the TWJ-SDA reaction [1], and the 5' end strand is the template strand for primer1 [2].

Figure 1. Mechanism of TWJ > 3step-SDA

Figure 2. The Result of TWJ > 3step-SDA

When TWJ-SDA was connected to 3step-SDA, an increase in amplification speed was observed around the 40-minute mark.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1]: Qing, Z., Feng, C.,Feng, X., Yongxi, Z., & Chunhai, F. (2014). Target-Triggered Three-Way Junction Structure and Polymerase/Nicking Enzyme Synergetic Isothermal Quadratic DNA Machine for Highly Specific, One-Step, and Rapid MicroRNA Detection at Attomolar Level. Anal. Chem. 2014, 86, 16, 8098-8105. https://doi.org/10.1021/ac501038r

[2]: Komiya, K., Noda, C. & Yamamura, M. (2024). Characterization of Cascaded DNA Generation Reaction for Amplifying DNA Signal.New Gener. Comput. 42, 237-252. https://doi.org/10.1007/s00354-024-00249-2